Courses organised by:
Molecular and Medical Microbiology Research Group
Dr Pamela Greenwell Dr Sanjiv Rughooputh
Research Co-ordinator Lecturer
Course Director Laboratory Manager
Laboratory Director Knowledge Transfer Fellow (WestFocus)
Knowledge Transfer Fellow (WestFocus) email@example.com
Websites : http://www.wmin.ac.uk/cavendish/bioshort.htm
September 2005 £200 (£180 for IBMS Members and RCPath trainees)
09.30 Welcome and coffee
10.00- 10.30 Introduction to practical: DNA isolation.
10.30- 11.30 Practical 1: PCR Detection of sickle cell disease using PCR and RFLP: setting up the PCR.
11.45-13.00 Lecture: PCR
14.00-15.00 Practical 2 Restriction enzyme digest of PCR product. Preparation of agarose gel
15.00-15.45 Lecture: Primer choice and complex PCR
15.45 –16.15 Practical 3 Run gels
16.15-17.15 Discussion: Utility of PCR in routine diagnostics
17.15-18.00 Practical: Visualise gels and discuss results
2: Real time PCR and Primer design One day laboratory/ Lecture
September 2005 £200 (£180 for IBMS Members, RCPath trainees, ABA members and those applying through the ABA)
09.30 Welcome and coffee
10.00- 11.00 Lecture: PCR and real time PCR theory. Detection chemistries
SYBR green, TaqMan and Molecular Beacons
11.00- 12.00 Practical 1: Quantitation of microorganisms
12.00-13.00 Lecture: Reverse transcriptase PCR , the use of housekeeping genes
14.00-15.00 Practical 3 View results
15.00-16.00 Lecture: Quantitation strategies
16.15 –17.15 Lecture: Primer design, BLAST and CLUSTALW
17.15-18.00 Practical: Design primers and check for specificity
A one day theory and practical session that gives you an insight of bioinformatics. With new sequence data being lodged everyday, bioinformatics is high on the agenda for serious scientists who want to advance in their field.
What is bioinformatics? ► Why is it useful? ► How can I make the best of the resources available?
1) Internet data resources
a) Effective interrogation of bibliographical database, accessing free journals and books, Pubmed.
b) Uses of the On line Mendelian Inheritance in Man (OMIM) database for the analysis of human inherited diseases
c) Accessing DNA and protein sequence information using genome, gene and protein databases
d) Specialist databases such as antibody, HLA, carbohydrate and disease specific resources
2) Data mining: finding , analyzing and using sequence data
a) Sequence searching and downloading
b) Comparisons between sequences using Blast and CLUSTALW. Uses of sequence comparisons in evolutionary studies, gene prediction and probe design.
c) Primer design using specialist software
d) Utilising sequence comparison tools to validate uniqueness and utility of primers and probes.
of monoclonal antibodies by hybridoma technology is a very labour intensive,
tedious and expensive process that does not guarantee success after all the
lengthy procedures. Phage display technology, offers a powerful
alternative to obtain target specific, genetically stable human
antibodies from combinatorial libraries. This 5 day intensive practical/
theory course will cover the essential processes in the production of monoclonal
antibodies by phage display. The participant will have the opportunity to
screen a library for their antibodies of choice to take away!
The course structure is as follows:
►Why phage display? : Phage display technology, Origin, Biology of filamentous phage Genome of m13 series of helper phage, Bacterial hosts and their roles and expression of antibodies in E.coli. Antibodies structure and function. ►Construction of combinatorial library from human and other animal sources: Principle of selection of ScFV, production of ScFv by Biopanning, ►Characterisation of ScFv, ELISA, DOT BLOT, Immunoblot and PCR
► Propagation of helper phage and phagemid ► Panning of library
►Elution of antibodies ► Infection of E.coli and growth ►Harvesting phage
►Production of soluble antibody ►ELISA, Dot blot and immunoblot ►PCR.
For details please contact Dr Pamela Greenwell (firstname.lastname@example.org )
Dr Sanjiv Rughooputh (email@example.com)
Or By phoning 0207 911 5000 ext 3690.