Courses organised by:
Molecular and Medical
Microbiology Research Group
115, New
Course Leaders:
Dr Pamela Greenwell Dr
Sanjiv Rughooputh
Research Co-ordinator Lecturer
Course Director Laboratory Manager
Laboratory Director Knowledge
Transfer Fellow (WestFocus)
Knowledge Transfer Fellow (WestFocus) rughoos@wmin.ac.uk
Websites
: http://www.wmin.ac.uk/cavendish/bioshort.htm
September
2005 £200
(£180 for IBMS Members and RCPath trainees)
09.30 Welcome and coffee
10.00- 10.30 Introduction to practical: DNA isolation.
10.30- 11.30 Practical 1: PCR Detection of sickle cell disease using
PCR and RFLP: setting up the PCR.
Break
11.45-13.00 Lecture: PCR
LUNCH
14.00-15.00 Practical 2 Restriction enzyme
digest of PCR product. Preparation of agarose gel
15.00-15.45 Lecture: Primer choice and
complex PCR
15.45 –16.15 Practical 3 Run gels
Break
16.15-17.15 Discussion: Utility of PCR in
routine diagnostics
17.15-18.00 Practical: Visualise gels and
discuss results
18.00 Refreshments
2: Real
time PCR and Primer design One
day laboratory/ Lecture
September
2005 £200 (£180 for IBMS Members, RCPath
trainees, ABA members and those applying through the ABA)
09.30 Welcome and coffee
10.00- 11.00 Lecture: PCR and real time PCR theory.
Detection chemistries
SYBR green,
TaqMan and Molecular Beacons
11.00- 12.00 Practical 1: Quantitation of microorganisms
12.00-13.00 Lecture: Reverse transcriptase
PCR , the use of housekeeping genes
LUNCH
14.00-15.00 Practical 3 View results
15.00-16.00 Lecture: Quantitation
strategies
16.15 –17.15 Lecture: Primer design, BLAST and CLUSTALW
17.15-18.00 Practical: Design primers and
check for specificity
18.00 Refreshments
A one day
theory and practical session that gives you an insight of
bioinformatics. With new sequence data
being lodged everyday, bioinformatics
is high on the agenda for serious scientists who want to advance in
their field.
What is
bioinformatics? ► Why is it useful? ► How can I make the best of
the resources available?
Practical
1) Internet data
resources
a) Effective
interrogation of bibliographical database, accessing free journals and books,
Pubmed.
b) Uses of the On line
Mendelian Inheritance in Man (OMIM) database for the analysis of human
inherited diseases
c) Accessing DNA and
protein sequence information using genome, gene and protein databases
d) Specialist databases
such as antibody, HLA, carbohydrate and disease specific resources
2) Data mining:
finding , analyzing and using sequence
data
a) Sequence searching and
downloading
b) Comparisons between
sequences using Blast and CLUSTALW. Uses of sequence comparisons in
evolutionary studies, gene prediction and probe design.
c) Primer design using
specialist software
d) Utilising sequence
comparison tools to validate uniqueness and utility of primers and probes.
25th July
Production
of monoclonal antibodies by hybridoma technology is a very labour intensive,
tedious and expensive process that does not guarantee success after all the
lengthy procedures. Phage display technology, offers a powerful
alternative to obtain target specific, genetically stable human
antibodies from combinatorial libraries. This 5 day intensive practical/
theory course will cover the essential processes in the production of monoclonal
antibodies by phage display. The participant will have the opportunity to
screen a library for their antibodies of choice to take away!
The course structure is as follows:
Theoretical
aspects
►Why phage display? : Phage display technology, Origin, Biology of
filamentous phage Genome of m13 series of helper phage, Bacterial hosts and
their roles and expression of antibodies in E.coli. Antibodies
structure and function. ►Construction of combinatorial library from human
and other animal sources: Principle of selection of ScFV, production of
ScFv by Biopanning, ►Characterisation of ScFv, ELISA, DOT BLOT, Immunoblot
and PCR
►
Propagation of helper phage and phagemid
► Panning of library
►Elution
of antibodies ► Infection of E.coli and growth ►Harvesting phage
Additional
practicals
►Production
of soluble antibody ►ELISA, Dot blot and immunoblot ►PCR.
For details please contact Dr Pamela Greenwell (greenwp@wmin.ac.uk )
Dr Sanjiv Rughooputh (rughoos@wmin.ac.uk)
Or By
phoning 0207 911 5000 ext 3690.